Abstracts

Bacterial Diversity in Surface Sediments from the Pacific Arctic Ocean

Huirong Li¹, Yong Yu², Wei Luo³, Yinxin Zeng⁴, Bo Chen^5
1Polar Biological Science Division, Polar Research Institute of China, 451 Jinqiao Road, Shanghai, 200136, China, Phone 86-21-58717207, Fax 86-21-58711663, lihuirong@pric.gov.cn
²Polar Research Institute of China, Shanghai, China
³Polar Research Institute of China, Shanghai, China
⁴Polar Research Institute of China, Shanghai, China
⁵Polar Institute of China, Shanghai, China

16S ribosomal DNA clone library analysis was performed to assess bacterial diversity within four surface sediment samples (0–5cm) collected from the Pacific Arctic Ocean. The nearly complete length of 16S rDNA was obtained for 463 clones from four libraries and 13 distinct major lineages of Bacteria were defined (α, β, γ, δ, and ε-Proteobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Actinobacteria, Firmicutes, Planctomycetes, Spirochaetes, and Verrucomicrobia). α, γ, and δ-Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria were common phylogenetic groups from all the sediments. Particularly, the γ-Proteobacteria was the dominant bacterial lineage, which represented near or over 50% of the clone libraries. Over 35% of δ-Proteobacteria clones of four clone libraries were closely related to cultured bacterial isolates with similarity values ranging from 94 to 100%. The community composition was different among sampling sites, which potentially was related to geochemical difference.

Abstract Categories: 1.1 Advances in Understanding Arctic System Components